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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Comp Neurol. 2010 Jun 1;518(11):1925–1945. doi: 10.1002/cne.22311

Figure 4. Diagram of 70 kB pdfr-myc P[acman].

Figure 4

Four steps used to generate the 70 kB pdfr-myc P[acman] flies. Step (1). Gap repair between linearized P[acman] and BACR25B3 at pdfr locus. Step (2). Recombination and insertion of a 6xMYC tag and Neomycin resisitance selection marker at the C terminus of pdfr. Step (3). Cre recombinase mediated removal of NeoR cassette. Step (4). φC31-mediated integration of 70 kB pdfr-myc P[acman] at attP2 site (681A-B2, Groth et al., 2004).