Figure 5. The 70 kB pdfr-myc transgene rescues pdfr behavioral defects.

Representative examples of circadian behavior of three independent experiments. (A–D) Averaged activity for 6 days in a 12 h:12 h LD cycle. (A) w¹¹¹⁸ control flies. (B) pdfr⁵³⁰⁴, a large deletion mutant of pdfr. (C) pdfr⁵³⁰⁴; ; att^P2, pdfr⁵³⁰⁴ with an empty docking site. (D) pdfr⁵³⁰⁴; ; pdfr-myc/+, pdfr⁵³⁰⁴ with a single copy of pdfr-myc. (E–H) Representative actograms of single flies of each genotype for 6 days in LD and 9 days in DD. Wild-type control w¹¹¹⁸ exhibits normal morning and evening peaks under LD and a free-running rhythm with a 23.5 hr period (A, E). A larger pdfr deletion (pdfr⁵³⁰⁴) produced an advanced evening peak and weak or no morning peak; under DD, ~50 % of pdfr⁵³⁰⁴ became arrhythmic and ~50% displayed short, weak rhythms of ~22 hr period (B, F). pdfr⁵³⁰⁴; ; att^P2 – control for the rescue experiment: pdfr⁵³⁰⁴ mutant flies containing the att^P2 docking site that lacks any pdfr sequences. This control construct cannot rescue the behavioral defects of either pdfr³³⁶⁹ (data not shown) or pdfr⁵³⁰⁴ (C, G). pdfr⁵³⁰⁴; ; pdfr-myc/+: 70 kB pdfr-myc transgene in pdfr⁵³⁰⁴ background. Both LD and DD behavioral defects of both pdfr mutants were rescued by the 70 kB pdfr-myc transgene (D, H) (compiled data is presented in Table 6).