Figure 6. PDF and PDFR-MYC expression.

Brains of w¹¹¹⁸; ; pdfr-myc flies were immunostained with anti-MYC (green) and anti-PDH (magenta) antibodies. (A) Schematic drawing of PDF-expressing neurons in the fly brain in frontal view. Dorsal is to the top and ventral to the bottom. Yellow inner boxes depict the region of images presented in panels (C) and (I). (B) Schematic drawing of PDF-expressing neurons in the fly brain in horizontal view. Anterior is to the top and posterior to the bottom. A yellow inner box depicts the region of images represented in panel in (F). (C–E) Z-series projections of dorsal brain imaged from the posterior aspect. (C) Merged image of (D) (MYC, green) and (E) (PDF, magenta). Red asterisks indicate the incidence of non-pacemaker receptor-positive cell bodies. (F–H) Sub-retinal glial cells express PDFR-MYC at the boundary of retina and lamina; and large LNv PDF is localized in distal medulla. (F) Merged image of (G) (MYC, green) and (H) (PDF, magenta). (I–K) Suboesophageal ganglion (SOG) with PDF-tri cells imaged from the anterior aspect. (I) Merged image of (J) (MYC, green) and (K) (PDF, magenta). Arrows, PDF-tri cell bodies. re, retina; la, lamina; me, medulla. Scale bars, 50μm.