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. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: J Neurochem. 2006 Apr 21;97(5):1301–1313. doi: 10.1111/j.1471-4159.2006.03797.x

Fig. 6.

Fig. 6

15-A2t-Isoprostane (15-A2t-IsoP) potentiates oxidative glutamate toxicity in neurons and HT22 cells. (a) Immature (1–2 DIV) primary cortical neurons were exposed to 1.5 mM glutamate or 5 μM 15-A2t-IsoP alone or in combination. Cell death was assessed after 18 h by lactate dehydrogenase (LDH) assay. (b) HT22 cells were exposed to glutamate plus increasing concentrations of 15-A2t-IsoP. Cell viability was assessed by MTT assay after 20–24 h. Note that in both (a) and (b), 15-A2t-IsoP and glutamate in combination resulted in significantly increased cell death. Data in (a) and (b) represent the mean ± SEM of three independent experiments, each performed in triplicate, and were analyzed by one-way ANOVA. *p < 0.05 vs. control.