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. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: Cancer Res. 2009 Jul 1;69(13):5490–5497. doi: 10.1158/0008-5472.CAN-09-0304

Figure 1.

Figure 1

Adoptive transfer of Treg cells in APCΔ468 mice causes polyps to regress and reduces mastocytosis. A, nonlinear regression of the number versus the diameter of polyps remaining after adoptive transfer of nTreg from healthy mice. Blue, control APCΔ468; green, 3 wk after adoptive transfer of 106 Treg cells; red, 6 wkafter adoptive transfer of 106 Treg cells. Adenomatous polyps in mice transferred with Treg cells display increased apoptosis and decreased proliferation. Mice were transferred with 1 × 106 nTreg cells derived from healthy C57BL/6 mice 6 wkbefore analysis. B, frequency of apoptotic nuclei and mitotic cells in polyps of APCΔ468 mice with no transfer (white columns) or after Treg transfer (gray columns). C, chloroacetate esterase staining of mast cells infiltrating the polyps in APCΔ468 (a) and in APCΔ468 6 wkafter adoptive transfer of 106 Treg cells (b). Magnification, ×200. c, mast cells per 200× field. White circles, APCΔ468; gray circles, APCΔ468 6 wkafter adoptive transfer of 106 Treg cells; black circles, wt. D, MCps assay using MNCs prepared from the intestine 6 wkafter adoptive transfer of 106 Treg cells. White column, APCΔ468; gray column, APCΔ468; black column, wt.