Figure 2.

Frequencies and numbers of CD4⁺CD25⁺Foxp3⁺ cells are elevated in APC^Δ468 mice. A, contour fluorescence-activated cell sorting (FACS) plots of CD4⁺CD25⁺Foxp3⁺ cells prepared from the MLN of (a) control C57BL/6 and (b) age-matched APC^Δ468 mice or intestine of (c) control C57BL/6 and (d) microdissected intestinal polyps of APC^Δ468 mice or (e) tissue marginal to polyps of APC^Δ468 mice. B, frequencies of CD4⁺CD25⁺Foxp3⁺ cells in C57BL/6 (black columns) and APC^Δ468 (white columns) mice. Lymph nodes from the neck, spleen, and MLN were analyzed from healthy intestine (black columns), microdissected polyps (white columns), and tissue marginal to polyps (gray column). C, total numbers of CD4⁺CD25⁺Foxp3⁺ cells in the spleen, MLN, and intestine of control C57BL/6 (black columns) and APC^Δ468 (white columns) mice. B6 mice (n = 7) and APC^Δ468 mice (n = 11).