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. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: Cancer Res. 2009 Jul 1;69(13):5490–5497. doi: 10.1158/0008-5472.CAN-09-0304

Figure 4.

Figure 4

CD4+Foxp3+ cells derived from polyp-ridden APCΔ468 mice are deficient in IL-10 but produce IL-17. MNCs isolated from MLN, spleen, intestine, microdissected adenomatous polyps, or healthy tissue from the margin of the polyps were cultured with anti-CD3/CD28 beads for 3 d and then stained for cell surface expression of CD4, CD25, intracellular Foxp3, and IL-10 (A) or IL-17 (B) and analyzed by FACS. All contour plots except for isotype control have been pregated for CD4. Representative contour plots from one of three independent assays are shown, depicting expression of IL-10 or IL-17 and Foxp3. Isotype controls are shown for IL-10 (IgG2b) and IL-17(IgG1).