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Effect of Methotrexate on the viability of different cancer cell lines using MTT assay
| Cell lines | Methotrexate IC50 (μg/ml) | Methotrexate IC50 (nmol) |
|---|---|---|
| L929 | 0.46 ± 0.03 | 101.2 |
| NIH3T3 | 0.24 ± 0.013 | 50 |
| T47D | 0.16 ± 0.09 | 31 |
| Caco2 | 0.23 ± 0.04 | 70.4 |
| HT29 | 0.23 ± 0.02 | 50 |
Methotrexate as an anticancer drug was used as a positive control. Data are mean ± SD of three separate experiments
