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. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: Insect Mol Biol. 2005 Apr;14(2):185–194. doi: 10.1111/j.1365-2583.2004.00547.x

Figure 5.

Figure 5

RT-PCR analysis. (A) Lanes 1, 2 and 3, total RNA from 1-, 3- and 5-day old larvae; lanes 4 and 5, total RNA from 0.5 and 12 h pupae; lane 6, total RNA from males; lane 7, total RNA from females; lanes 8, 9 and 10, total RNA from females at 24, 36 and 48 h after blood feeding. (B) Lanes 1, 2, 3 and 4, total RNA from unwashed head, thorax, abdomen and ovaries, and lanes 5, 6, 7 and 8, total RNA from head, thorax, abdomen and ovaries that had been extensively washed before total RNA isolation. (C) Lane 1, total RNA from haemocytes; lanes 2 and 3, total RNA from unwashed and washed ovaries, respectively; lanes 4 and 5, RT-PCR results for chorion peroxidase (CPO) amplified from total RNA of unwashed and washed ovaries; lanes 6 and 7, RT-PCR results for dopa decarboxylase (DDC) amplified from total RNA of unwashed and washed ovaries. In (C), the same unwashed or washed sample was used for amplification of Ae-proPO-5, CPO and DDC.