Skip to main content
. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: Immunogenetics. 2007 Apr 27;59(7):525–537. doi: 10.1007/s00251-007-0222-x

Fig. 1.

Fig. 1

Spectral data (left) and cluster plots (right) illustrating single nucleotide and double nucleotide calls with the hME assay “3DS1.3DL1.D1.S,” which discriminates between KIR3DS1 and KIR3DL1. a and c show spectral data and cluster plots for the single nucleotide call T (3DL1) and G (3DS1), respectively. b shows data for the double nucleotide call T/G (KIR3DL1 and KIR3DS1 both present). Arrows indicate specific data points illustrated in spectral graphs. For all panels, the lowest mass peak (6447.2 Da) represents the unextended primer. The peak produced when the polymerase pauses leading to incorporation of an unexpected dNTP (pausing peak) is also shown—Cluster plots (right) illustrate the intensity of the high mass product (T) peak vs intensity of the low mass product (G) peak for a given sample. The separation between clusters and the tightness within a cluster are indicative of the accuracy and specificity of the assay