. Author manuscript; available in PMC: 2010 Jun 7.

Published in final edited form as: Immunogenetics. 2007 Apr 27;59(7):525–537. doi: 10.1007/s00251-007-0222-x

Table 5.

Concordance between KIR/MALDI genotyping and established methods: results for 15 samples at 17 KIR loci

		2DL1	2DL2	2DL3	2DL4	2DL5 generic	2DL5 A&B	3DL1	3DL2	3DL3	2DS1	2DS2	2DS3	2DS4 generic	2DS4 *del	2DS5	3DS1	2DP1	3DP1

Number of probes/locus		3	4	2	2	2	5	2	2	2	2	2	2	2	1	2	3	1	1
BM16	Exp	+		+	+			+	+	+				+				+	+
	Obs	+		+	+			+	+	+				+	+			+	+
PITOUT	Exp	+	+	+	+			+	+	+		+		+				+	+
	Obs	+	+	+	+			+	+	+		+		+	+			+	+
BM92	Exp	+	+	+	+	+		+	+	+	+	+	+	+		+	+	+	+
	Obs	+	+	+	+	+	A+B	+	+	+	+	+	+	+		+	+	+	+
OLGA	Exp	+		+	+	+	A+B	+	+	+	+			+		+	+	+	+
	Obs	+		+	+	+	NEW	+	+	+	+			+	+	+	+	+	+
RML	Exp	+	+	+	+	+	A	+	+	+	+	+		+		+	+	+	+
	Obs	+	+	+	+	+	A	+	+	+	+	+		+		+	+	+	+
HS	Exp	+		+	+			+	+	+				+	+			+	+
	Obs	+		+	+			+	+	+				+	+			+	+
NV	Exp	+	+		+	+	A+B	+	+	+	+	+	+	+		+	+	+	+
	Obs	+	+		+	+	A+B	+	+	+	+	+	+	+		+	+	+	+
WC	Exp	+	+	+	+	+		+	+	+	+	+		+	+	+		+	+
	Obs	+	+	+	+	+	B	+	+	+	+	+		+	+	+		+	+
WT47	Exp	+	+		+	+	A+B		+	+	+	+	+			+	+	+	+
	Obs	+	+		+	+	A+B		+	+	+	+	+			+	+	+	+
RR	Exp	+	+	+	+	+	B	+	+	+		+	+	+	+			+	+
	Obs	+	+	+	+	+	B	+	+	+		+	+	+	+			+	+
YW	Exp	+		+	+			+	+	+				+	+			+	+
	Obs	+		+	+			+	+	+				+	+			+	+
T7527	Exp	+	+	+	+	+		+	+	+	+	+	+	+			+	+	+
	Obs	+	+	+	+	+	B	+	+	+	+	+	+	+			+	+	+
HOR	Exp	+		+	+	+	A		+	+	+					+	+	+	+
	Obs	+		+	+	+	A		+	+	+					+	+	+	+
DU145	Exp		+		+	+	A		+	+	+	+				+	+		+
	Obs		+		+	+	A		+	+	+	+				+	+		+
FC	Exp	+	+		+			+	+	+		+	+	+	+			+	+
	Obs	+	+		+			+	+	+		+	+	+	+			+	+

Abbreviations : Exp = expected; Obs = observed

A “+” in the expected row signifies that the locus is expected to be present; blank signifies that locus is not expected to be present. Filled gray indicates that the sample had not been previously typed at allelic level resolution. “NEW” is a putative novel allele found for “OLGA” at KIR 2D L 5 A/B .

Expected KIR genotypes for BM16, PITOUT, BM92, OLGA, RML, T7527 and HOR are as shown in Cook et al. 2003. Expected KIR genotypes for RML, WT47, OLGA, HOR, NV, and DU145 are as shown in Gomez-Lazano and Vilches 2002 and 2004. Expected KIR genotypes for HS, WC, RR, YW and FC are unpublished results from PP.

A generic assay for the presence of KIR 2DL 5 is combined with a higher resolution assay capable of resolving KIR 2DL 5A and KIR 2DL 5B . A generic assay for KIR 2DS 4 is combined with an assay for detection of the deletion variants KIR 2DS 4*003 /*004/*006.