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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Immunol. 2009 Nov 30;184(1):67–72. doi: 10.4049/jimmunol.0903118

FIGURE 4.

FIGURE 4

Recognition of cells transfected with the vaccine plasmid encoding Rev. We transfected 721.221 cells that stably express either Mamu-A*02 or Mamu-B*08 with the plasmid encoding Gag from the vaccine, the Rev plasmid, the Rev plasmid engineered to express an escaped version of RY8, or an Env-encoding plasmid. At 24 h after transfection, we used ICS to see if T cell lines specific for Env788–795RY8 or Rev44–51RL8 could recognize the cells, as measured by TNF-α and IFN-γ production. We tested for recognition by RY8-specific cells grown from an animal vaccinated in this study (RY8 Vacc.), RY8-specific cells grown from an SIV-infected animal from a previous study (RY8 non Vacc.), and RL8-specific cells grown from an SIV-infected animal from a previous study (RL8).