. Author manuscript; available in PMC: 2010 Aug 14.

Published in final edited form as: J Mol Biol. 2009 Jun 6;391(2):461–470. doi: 10.1016/j.jmb.2009.05.084

Table 1.

Stability and m-values for RNase H variants. The values in parentheses represent the standard deviation calculated from at least three individual denaturant melts. The reported ΔG_UN for D10A/I25A was obtained by fixing the m-value to 2.1 kcal mol^-1 M^-1. See accompanying manuscript for details.

	wt⁶	I53A⁵	D10A⁵	I25A		D10A/I25A
				urea melt	model
Δ G_UN (kcal mol^-1)	9.7	7.6	13	3.4 (0.3)	4.5	7.86 (0.07)
m_UN (kcal mol^-1 M^-1)	2.1	2.1	2.1	1.3 (0.1)	2.1^*	2.1
Δ G_UI (kcal mol^-1)	3.55	1.66	4.38	ND	3.55^*	ND
m_UI (kcal mol^-1 M^-1)	1.24	1.24	1.45	ND	1.24^*	ND

Wild type values assumed for the model

ND: not determined