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SC associates with polymeric IgA2 b12. Microtiter plates were coated with polyclonal goat anti-human IgA Abs, and then overlaid with human secretory IgA (positive control), monomeric IgA2 b12 (negative control), or dimeric IgA2 b12 from CHO cell clones J2 or J10. The plates were then probed with human SC and then developed using anti-human SC Abs. Each data point represents the average of two microtiter wells tested in parallel.
