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. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: J Immunol. 2007 Sep 1;179(5):3144–3152. doi: 10.4049/jimmunol.179.5.3144

FIGURE 5.

FIGURE 5

Neutralization of HIV-1 infection of PBMCs by IgA2 b12. Monomeric, dimeric, and polymeric (i.e., a mixture of monomers, dimers, and higher molecular mass polymers) IgA2 b12, as well as IgG1 b12, at indicated concentrations were incubated with (A) HIV-1BaL or (B) HIV-1IIIb for 30 min before being mixed with activated PBMCs. Levels of p24 in PBMC culture supernatants were determined 6 days later by ELISA, as described in Materials and Methods. Viral p24 in the control wells were consistently >14 ng/ml, confirming that the control cells were productively infected with HIV. The mAb concentration (micrograms per milliliter) necessary to inhibit 50% of viral infection, referred to as the IC50, is indicated in the inset.