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. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: J Immunol. 2007 Sep 1;179(5):3144–3152. doi: 10.4049/jimmunol.179.5.3144

FIGURE 8.

FIGURE 8

Inhibition of HIV-1 BaL internalization in ME180 cells by b12 mAbs. Increasing concentrations of IgG1 or IgA2 b12 were mixed with HIV-1BaL before application to ME180 cells. After 1 h at 37°C, the cells were washed to remove unbound virus and then lysed. Levels of p24 Ag in cell lysates were considered representative of the total amount of virus bound specifically to cell surfaces, plus the amount of virus that had been internalized (□). Alternatively, ME180 cells were treated with trypsin-EDTA (0.05%) for 8 min before lysis to effectively remove virus bound to the cell surfaces. Levels of p24 Ag in cell lysates from trypsin-treated cells were considered representative of the only amount virus internalized (■).