Skip to main content
. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: J Immunol. 2007 Sep 1;179(5):3144–3152. doi: 10.4049/jimmunol.179.5.3144

Table I.

Neutralization of HIV-1-pseudotyped virus in luciferase reporter assays

Virus
JR-FL JR-CSF HxB2
mAb (nM)a IC90b IC50c IC90 IC50 IC90 IC50
 IgG1 1.3 0.7 5.3 0.7 0.7 <0.27
 mIgA2 4.1 0.6 >58.8 2.4 0.6 <0.24
 dIgA2 1.1 0.3 31.3 1.1 0.6 <0.11
 pIgA2 1.3 0.3 25.0 0.6 0.3 <0.11
 SIgA2 0.9 0.2 16.7 0.2 0.2 <0.1
mAb (μg/ml) IC90 IC50 IC90 IC50 IC90 IC50
 IgG1 0.2 0.1 0.8 0.1 0.1 <0.04
 mIgA2 0.7 0.1 >10 0.4 0.1 <0.04
 dIgA2 0.4 0.1 10.0 0.4 0.2 <0.04
 pIgA2 0.4 0.1 9.1 0.2 0.1 <0.04
 SIgA2 0.4 0.1 7.2 0.1 0.1 <0.04
a

The following molecular masses were used to calculate the molar concentration of each Ab: IgG1 150 kDa; mIgA2 170 kDa; dIgA2 (with J chain) 360 kDa; SIgA2 b12 (with J chain and SC) 430 kDa. The molecular mass of pIgA2 b12, which is a mixture of monomer, dimer, and higher molecular mass molecules, was assumed to be 320 kDa, the dominant species expressed by clone 1 expressing pIgA2 b12 as determined by gel filtration chromatography.

b

Concentrations of Ab required to neutralize 90% of the indicated virus, presented in nanomoles or micrograms per milliliter.

c

Concentrations of Ab required to neutralize 50% of the indicated virus, presented in nanomoles or micrograms per milliliter.