Table 1. Quantification of the von Kossa staining in cultures established from dental pulp cells, bone marrow and calvaria from P5-7 and P18-21 mice at different time points.
von Kossa staining was performed at day 10, 14 and 21. Area of the culture plate covered with stained mineralized tissue was quantified using Photoshop and ImageJ programs. Numbers represent the total area in mm2 of cultures stained with von Kossa. The numbers in parenthesis represent the percentage of area of von Kossa staining within the culture dish. Values represent mean ± SE from duplicate plates from three independent experiments.
| MOLAR PULP | BONE MARROW | CALVARIA | |||
|---|---|---|---|---|---|
| P5-7 | P18-21 | P5-7 | P18-21 | P5-7 | |
| day 10 | 53.1 ± 0.4 (13.9 ± 0.1%) | 0 | 17.8 ± 0.2 (4.6 ± 0.1%) | 12.6 ± 0.5 (3.3 ± 0.1%) | 0 |
| day 14 | 317.7 ± 0.3 (82.9 ± 0.1%) | 4.0 ± 0.8 (1.0 ± 0.2%) | 93.6 ± 1.2 (24.4 ± 0.3%) | 81.8 ± 9.0 (21.3 ± 2.3%) | 33.5 ± 1.1 (8.7 ± 0.3%) |
| day 21 | 360.0 ± 0.7 (93.8 ± 0.2%) | 30.4 ± 0.5 (7.9 ± 0.2%) | 260.3 ± 22.5 (68 ± 6%) | 296.1 ± 22.0 (77.3 ± 5.7%) | 133.0 ± 0.9 (34.7 ± 0.2%) |