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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: J Immunol. 2010 Mar 8;184(8):4362–4367. doi: 10.4049/jimmunol.0903142

FIGURE 4.

FIGURE 4

Comparison of binding of [3H]LOS to HEK293T cells transiently expressing full-length wt (●) or D299G.T399I (△) TLR4 with (A) or without (B) MD-2. Binding of [3H]LOS to transiently transfected HEK293T cells during 30 min incubation with increasing concentrations of [3H]LOS·sCD14 (A) or [3H] LOS·MD-2 (B) was measured as described in Materials and Methods. The results are expressed as percent of the maximum [3H]LOS binding measured (0.11 ± 0.02 pmol bound/5 × 106 cells in A and 0.13 ± 0.02 pmol bound per 5 × 106 cells in B after subtraction of the dose-dependent binding of [3H]LOS to mock-transfected cells. The results shown are the mean ± SEM of two (A) or three (B) independent experiments, each in duplicate.