Figure 9. Na+ influence on OAD secondary structure and on the ability to bind oxomalonate.

a. OAD secondary structure in the absence of NaCl (red line) and after the addition of 250 mM NaCl (blue line). b. OAD secondary structure in the absence NaCl before (red full line) and after addition of 10 µM oxomalonate (black dotted line). OAD was purified in 250 mM KCl, pH 7.4, 0.5% Tween 20, 0.05% Brij 58, 100 mM KH₂PO₄ buffer.