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Mapping of the binding domains for DDB1 and Cdh1. A, schematic description of the domains of Cdh1 and DDB1. B, the indicated plasmids of Cdh1 were cotransfected with V5-DDB1 (n = 3). C and D, the indicated constructs of DDB1 were cotransfected with Myc-Cdh1; and the formation of immunoprecipitation (IP) complex (B, C, and D) was detected as described in Fig. 1 (n = 3). WCL, whole cell lysate; aa, amino acid; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; CB, c-box; IR, IR-tail.
