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. 2010 Apr 8;285(24):18252–18261. doi: 10.1074/jbc.M109.080382

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Yet1p and Yet3p form a complex and interact with Sec63p and Sec61p. A, MYC immunoprecipitations were performed on digitonin-solubilized microsomes from untagged (YPH500) and Yet1p-MYC (CBY0310) strains. Bound material was resolved by SDS-PAGE and silver stained. Arrows indicate bands that were excised (from a colloidal Coomassie-stained gel run in parallel, not shown) and identified by mass spectrometry. The line indicates a prominent band presumed to be Yet1p-MYC. B, HA immunoprecipitations were carried out on digitonin-solubilized semi-intact cells from untagged (BY4742), Yet1p-HA (CBY2795), and Yet3p-HA (CBY2805) tagged strains and analyzed by immunoblot. C, HA immunoprecipitations performed and analyzed as in B from untagged (BY4742), Yet3p-HA (CBY2805), Yet3pΔCT-HA (CBY2815), and Yet3p-HA yet1Δ (CBY2810). Immunoblots for the ER resident protein Sec12p are included as a negative control for immunoprecipitations and is indicated by an arrow.