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. 2010 Apr 8;285(24):18252–18261. doi: 10.1074/jbc.M109.080382

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Influence of sec71Δ mutation on Yet-Sec complex interaction. MYC and HA immunoprecipitations were performed and analyzed as described in the legend to Fig. 3B from: A, Yet1p-MYC (CBY2291), Yet1p-MYC sbh1Δ (CBY2292), Yet1p-MYC sec71Δ (CBY2293) and Yet1p-MYC sec72Δ (CBY2363); B, Yet3p-HA (CBY2805) and Yet3p-HA sec71Δ (CBY2813); C, no tag (BY4742), Sec63p-HA (CBY2733), and Sec63p-HA sec71Δ (CBY2814). Immunoblots for the ER resident protein Sec12p are included as a negative control and noted with an arrow.