Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Apr 8;285(24):18252–18261. doi: 10.1074/jbc.M109.080382

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Yet-Sec complex interaction is enhanced by DTT-induced ER stress. A, semi-intact cells from untagged (CBY2615) or Yet3p-HA (CBY2647) strains were produced from log phase cells treated with or without 8 mm DTT for 2 h prior to harvest. HA immunoprecipitations were performed and analyzed from these semi-intact cells as described in the legend to Fig. 3B. B, semi-intact cells from untagged (BY4742) and Sec63p-HA (CBY2733) strains were generated and used for HA immunoprecipitations as in A. For both A and B, immunoblots were quantified and immunoprecipitation efficiency of each protein was calculated by dividing the amount in the IP lane by the amount in the respective Input lane. Data are plotted as the mean of the ratio of +DTT IP efficiency over −DTT IP efficiency and are normalized to an IP efficiency of unity for Yet3p-HA (A) or Sec63p-HA (B). Error bars represent the S.D. with n = 3 except n = 5 for Yet complex (n = 3 for Yet1p and n = 2 for Yet3p) in B.