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Cyclin B2 is a transcriptional target of menin. A, four amplicons were used to detect the indicated regions of the Ccnb2 promoter for ChIP assay; n = 3. B, shown is a ChIP assay using menin antibody for Men1−/− and Men1+/+ MEFs (left) or the cells treated with 0.2 μg/ml nocodazole for 22 h (right); n = 3. C, shown is a ChIP assay using the E2F2 antibody (left) or the E2F3 antibody (right); n = 3. D, shown is a ChIP assay using anti-Pol II antibody (left) or control IgG (right); n = 3.
