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. 2010 Apr 19;285(24):18291–18300. doi: 10.1074/jbc.M110.106575

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Menin mutants A242V and L22R fail to reduce the level of acetylated histone H3 at the Ccnb2 locus. A, shown is a ChIP assay using menin antibody in Men1^−/− MEFs expressing control vector, menin, A242V, or L22R, n = 3. B, shown is a ChIP assay using acetylated-histone H3 antibody in the indicated cells, n = 3. C, shown is a ChIP assay using histone H3 antibody to see the level of occupancy of the histone H3 at the Ccnb2 locus in the indicated cells, n = 3. D, shown is a ChIP assay using IgG antibody as a control in the indicated cells, n = 3. *, p < 0.05; **, p < 0.01.