FIGURE 1.

Cloning and refolding of recombinant EhCP4. A, EhCP4 was cloned as a fusion protein with bacterial thioredoxin A (underline) with the His tag (underline) at the amino terminus of the prodomain of EhCP4 (dotted line). The mature enzyme is highlighted with gray in the box. B, time course of autocatalytic processing of rEhCP4 (Coomassie Blue stain). C, a Western blot shows formation of active enzyme with its active site labeled by the activity-based cysteine proteinase probe, DCG04. *, location of the mature enzyme on the gels.