FIGURE 2.

Optimal pH values of proteolytic activity and autocatalytic processing of rEhCP4. A, relative activities of rEhCP4 at different pH values are shown (the highest activity, set as 100%). The optimal pH of catalytic activity of rEhCP4 was pH ∼7. B, effect of pH on the autocatalytic processing of pro-rEhCP4 to produce mature enzyme (relative to the highest activity as 100% when assayed at pH 7). Data were collected from three experiments. p values, comparing multiple pH values, were calculated by two-way analysis of variance, and p values between two pH values were calculated using Student's t test. Error bars, S.E.