FIGURE 4.

Collagen deposition and neutrophil infiltration of liver samples from mice fed the MCD, MD, or CD diet. A, samples from mice fed the different diets for 15 days were stained with Sirius red to monitor collagen deposition. Please note that mice fed the MCD, MD, but not CD diet exhibit higher collagen deposition reflected by increased red fibers. B, parallel samples from mice fed the different diets were processed for MPO staining, indicative of neutrophil infiltration and inflammation. As with the Sirius red staining, mice fed the MCD, MD, but not CD diet display MPO staining. Moreover, some mice were fed the MCD diet and treated with GSH-EE intraperitoneally twice a week and then processed for Sirius red and MPO staining, indicating that GSH-EE ameliorated the extent of collagen accumulation and inflammation compared with mice. Images are representative of five to seven individual mice showing similar findings.