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Hepatic and mitochondrial SAM levels from liver samples of mice fed the MCD, MD, or CD diet. A, liver samples from the different nutritional groups were homogenized and processed for SAM determination by HPLC as described under “Experimental Procedures.” B, alternatively, samples were fractionated to isolate mitochondria and processed for SAM determination as in A. Results are the mean ± S.D. (error bars) of four to six individual mice. *, p < 0.05 versus control mice.
