FIGURE 3.

Molecular sieve chromatography of CNBr-digested human articular cartilage collagen. The CNBr digest of cartilage residue after 4 m guanidine HCl extraction was chromatographed on an agarose A1.5m (Bio-Rad) molecular sieve column (170 × 1.5 cm), eluted with a 0.05 m Tris-HCl buffer, pH 7.5, containing 2 m guanidine HCl, at a flow rate of 6 ml/h, collecting 3.0-ml fractions. Aliquots of collected fractions (4 μl) were assayed for mAb 4G9 immunoreactivity. The result shows that the N-propeptide domain of the type III collagen was retained in the cartilage matrix.