FIGURE 3.

Effect of SK inhibitor on the proliferation of A2780 and A2780/HPR cells and on HPR sensitivity in A2780/HPR cells. A, 12 h after seeding, A2780 and A2780/HPR cells were treated with SK inhibitor (gray), and cell viability was evaluated after 96 h as mitochondrial metabolic activity measured by the MTT reduction assay. Data are expressed as percentage of the control treated with vehicle (white). B, 12 h after seeding, A2780/HPR cells were treated with SK inhibitor in the presence of different HPR concentrations and, after 96 h, the mitochondrial metabolic activity was measured by the MTT reduction assay (B, left), and cell number was determined by counting trypsinized cells using a Bürker counting chamber (B, right). Data are the means ± S.D. of three different experiments. *, p < 0.001 versus controls, cells treated with vehicle only.