FIGURE 2.

UNG2 is poorly expressed in the natural target cells of HIV-1. UNG2 expression levels in two primary cell subsets (PBMCs and MDMs) and two laboratory cell lines (293T and TZM-bl) were measured by quantitative RT-PCR for UNG2 mRNA (A) and immunoblot analysis using the UNG2-specific antibody Ab225. Blots were also probed with an anti-α-tubulin antibody as a loading control. Independent blood donors are labeled #1 and #2 (B). Data shown are means (±S.E.) from independent experiments or independent blood donors for PBMCs and MDMs.