UNG2 in CCR5- and CXCR4-expressing primary T-lymphocytes. Freshly isolated PHA-stimulated PBLs were stained for CCR5, CXCR4, and UNG2. UNG2 expression levels in the total, CCR5-expressing, and CXCR4-expressing cell populations was analyzed by FACS (A). Freshly isolated PHA-stimulated PBLs were either mock nucleofected, nucleofected with a non-silencing control siRNA (siRandom), or nucleofected with a UNG2-specific siRNA (siUNG2). 48 h post infection cells were stained for CCR5, CXCR4, and UNG2. UNG2 expression levels in the total, CCR5-expressing, and CXCR4-expressing cell populations was analyzed by FACS (B). Data shown are means (±S.E.) from four independent blood donors.