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. 2010 Apr 6;285(24):18603–18614. doi: 10.1074/jbc.M109.090126

FIGURE 6.

FIGURE 6.

HIV-1 infection induces a transient increase in UNG2 expression in primary T-lymphocytes. Freshly isolated PHA stimulated PBLs were infected with equivalent amounts, as determined by HIV-1 p24 (CA) protein, of either NL4.3 or NLA8 HIV-1 virus. Cells were harvested 6 and 12 h post infection (A) or 48 h post infection (B) and UNG2 mRNA expression levels were measured by quantitative RT-PCR and normalized to GAPDH copy numbers. HIV-1 mRNA expression levels 48 h post infection were measured by quantitative RT-PCR (C). UNG2 mRNA levels normalized to HIV-1 RNA copies 48 h post infection (D). Freshly isolated PHA-stimulated PBLs were incubated with either RANTES or SDF1α at either 10 or 20 ng/ml. 12 h post addition of chemokines, cells were fixed and stained for CCR5 and UNG2. UNG2 expression levels in the total and CCR5-expressing cell populations was analyzed by FACS (E). Data shown are means (±S.E.) from either 4 (A–D) or 2 (E) independent blood donors. F, model of host cell UNG2 expression during HIV infection by X4 and R5 HIV.