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. 2010 Mar 19;285(24):18662–18671. doi: 10.1074/jbc.M110.108597

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — RP-HPLC-TOF/MS analysis of native and the variants after 1-day (a) and 4-day (b) incubation with H₂O₂. Following incubation with H₂O₂, the samples were analyzed by RP-HPLC-TOF/MS under nonreducing conditions at 215 nm on a Zorbax SB-300 C8 column. Note that the His/Ala variant released a significant amount of LC (peak 1), and as a result, a partial IgG1 molecule (HHL) missing one LC was observed at a much greater level in the His/Ala variant compared with other His variants as indicated by an arrow. In the Lys variants, a partial form of the IgG1 missing the Fab domain (Fab-Fc, peak 2) and the Fab domain (peak 3) were observed.