TABLE 1.

Summary of nonreducing CE-SDS results from the native and seven His²²⁹ and Lys²²⁷ variants, following 4-day H₂O₂ incubation

The component labels are as described for Fig. 6. The sum of the species is slightly less than 100% for most samples because of minimal levels of unclassified peaks with migration times of ∼18, 23, and 27 min in these samples. These species were not accounted for in the table because of their low amounts and are likely attributed to degradation products generated during sample preparation, as described by Michels et al. (38).

Sample	L	Fd	Fab	Fab-Fc	HH	HHL	Whole
				%
Control	0.3					0.7	99.0
K227A	26.9	8.2	7.6	12.2	23.3	15.5	2.0
K227Q	24.1	7.0	8.1	11.6	21.0	19.5	5.0
K227S	26.2	7.7	7.6	11.1	23.1	17.7	3.3
Native	29.7	4.8	7.6	8.7	33.6	12.1	1.2
K227S/H229S	28.5	1.9	2.7	4.3	35.0	21.4	3.0
H229A	32.5	2.2	3.3	4.0	46.1	9.1	0
H229Q	28.4	1.9	2.2	3.9	40.6	18.6	1.5
H229S	26.0	1.7	2.3	3.8	35.3	23.1	5.3