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. 2010 Apr 7;285(24):18806–18816. doi: 10.1074/jbc.M109.098079

FIGURE 1.

FIGURE 1.

Cool-1 is necessary for the maximal rate of migration of v-Src-transformed NIH 3T3 cells. Viral Src-transformed NIH 3T3 cells were transfected with two different siRNAs targeting Cool-1 (RNAi 1 and RNAi 2) or with control RNA. The cells were cultured for 24 h and then scored for their ability to migrate. Top-left panel, six different fields of each filter were photographed and the total number of cells that migrated through the filter were counted. The number of cells counted for the control were set at 100%. At least 200 cells were examined for each condition. The results from three experiments were averaged together and plotted. The error bars indicate ± S.D. Top-right panels, cell lysates were analyzed to assess the extent of the knockdown of Cool-1 expression by RNAi. Bottom panels, microscopic images of the cell migration assays shown in the top-left panel. The cells are visualized by GIEMSA staining (dark purple). The clear circles are not cells but represent holes in the filters.