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ATPase/helicase activities of wild type NS2B18NS3 (WT), its mutants, and NS3 helicase domain protein
| Construct | Km(ATP) | kcat (ATP) | ATPase activity kcat/Km as % of WT | Helicase activity (as % of WT) |
|---|---|---|---|---|
| μm | s−1 | |||
| NS2B18NS3 | 77 ± 6 | 14.3 | 100 | 100 |
| NS3 helicase | 106 ± 5 | 6.0 | 31 | 29 ± 8 |
| E173GP174 | 131 ± 5 | 10.9 | 45 | 33 ± 7 |
| P174G | 137 ± 6 | 11.1 | 44 | 68 ± 19 |
