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Expression of NAT8 in HEK293T cells. Western blot analysis of extracts (20 μg of total protein per lane) from HEK293T cells transfected for 48 h with empty pEF6-HisB (lanes 1–3), pEF6-NAT8 (lanes 4–6), and pEF6/Myc-NAT8 (lanes 7–9). The anti-His antibody used was the penta-His antibody (Qiagen) that detects both N- and C-terminally tagged NAT8s but with different affinities. Triplicates are the result of three independent transfection experiments.
