FIGURE 6.

CCNAT activity of human wild-type (WT) and mutant NAT8s overexpressed in HEK293T cells. a, specific CCNAT activity was assayed in cell extracts of HEK293T cells overexpressing wild-type (Glu-104/Phe-143) or mutant NAT8 proteins with a His₆ tag in the N or C terminus. b, relative expression levels of the mutant NAT8 proteins versus the wild-type NAT8 were evaluated by Western blot analysis using the anti-His antibody from GE Healthcare (to detect N-terminal tagged NAT8s) and the penta-His antibody from Qiagen (to detect C-terminally tagged NAT8s). c, specific CCNAT activity of each extract was corrected taking into account the differences in the expression for all NAT8 proteins.