FIGURE 5.
p22 contributes to cell growth in PF T. brucei cells. The RNAi vector p2T7-177-p22 was transfected into the PF strain 29-13 of T. brucei. RNAi was induced by the addition of 2.5 μg/ml tc to the cell media. A, 1 × 106 cells grown either in the absence (uninduced) or presence (induced) of tc were pelleted and immunoblotted for the presence of p22. The abundance of the mitochondrial RBP16 protein was analyzed as a control. B, cells grown either in the absence (−TET, filled diamonds) or presence (+TET, open diamonds) of tc were counted every 2 days, and cumulative cell numbers are indicated.