FIGURE 2.

Nine fragments of rec.c-gelsolin generated by rec.gzmB are identified by LC/ES-MS. A, observed and calculated/theoretical masses of the identified fragments of rec.c-gelsolin are listed as well as the possible assignments and flanking AA of the used rec.c-gelsolin or of the published AA sequence of mouse c-gelsolin. B, the six calculated gzmB cleavage sites of rec.c-gelsolin are listed (bold face). C, a schematic representation of the cleavage products of rec.c-gelsolin, observed by LC/ES-MS analysis, is depicted as lines in relation to the full-length gelsolin molecule. The NH₂- and COOH-terminal AA of each fragment as well as the molecular mass of the fragment are shown. The previously determined cleavage site of caspase 3 is depicted above the diagram.