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. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: J Cell Physiol. 2009 Dec;221(3):707–715. doi: 10.1002/jcp.21910

Fig. 2. km23-1 and km23-2 siRNAs selectively and specifically knock down both exogenous and endogenous km23-1 and km23-2.

Fig. 2

A: Left panel: 293T cells were transiently transfected with either EV (lane 1), or km23-2-Flag together with NC siRNA (lane 2), km23-1 siRNA (lane 3) or km23-2 siRNA (lane 4). Top panel, blockade of km23-1-Flag expression was analyzed via Western blotting with an anti-Flag M2 Ab. Bottom panel, equal loading was confirmed by blotting with an anti-DIC Ab. Right panel: 293T cells were transiently transfected with either EV (lane 1), or km23-1-Flag together with NC siRNA (lane 2), km23-1 siRNA (lane 3) or km23-2 siRNA (lane 4). Top panel, blockade of km23-1-Flag expression was analyzed via Western blotting with an anti-Flag M2 Ab. Bottom panel, equal loading was confirmed by blotting with an anti-DIC Ab. B: HaCaT cells were transfected with NC, km23-1-, or km23-2-specific siRNAs. Top panel, RT-PCR analysis of human km23-1 (left panel), and human km23-2 (right panel) mRNA expression from HaCaT cells were performed. Bottom panel, GAPDH was used as a loading control. Results are representative of two experiments.