Skip to main content
. Author manuscript; available in PMC: 2010 Jun 7.
Published in final edited form as: J Cell Physiol. 2009 Dec;221(3):707–715. doi: 10.1002/jcp.21910

Fig. 4. TGFβ induction of p21 expression in HaCaT cells specifically requires km23-2, but not km23-1.

Fig. 4

HaCaT cells were transiently transfected with the siRNA plasmids indicated: mock (lanes1-2), NC siRNA (3-4), km23-1 siRNA (lane 5-6), km23-2 siRNA (lane 7-8) and were treated with TGFβ as described in Fig. 3A. Top panel, p21 expression was detected by Western blot analysis using a mouse monoclonal anti-p21 Ab. Bottom panel, equal loading and expression of DIC was confirmed by blotting with a mouse monoclonal DIC Ab. Results are representative of two experiments.