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. 2010 Apr 28;30(17):6048–6057. doi: 10.1523/JNEUROSCI.5094-09.2010

Figure 5.

Figure 5.

D2R-stimulated hDAT A559V N-terminal phosphorylation promotes a channel-like mode of DAT. A, Representative traces of DAT-mediated channel-like activity in hDAT A559V, hDAT, hDAT A559V S/A, and hDAT S/D cells. hDAT A559V cells were also treated with raclopride (1 μm) and antCaMKIINtide (5 μm) as described above. Traces shown both for control condition (CTR) and after bath application of 10 μm cocaine (COC) to confirm DAT specificity. Isolated membrane patches were excised using the outside-out patch-clamp technique and held at −20 mV. B, Data were quantified using amplitude histogram analysis of 30 s of data to calculate the NPo of the DAT channel-like mode. For both CTR and COC conditions, data are reported as mean NPo ± SEM calculated for hDAT A559V cells (filled bars; n = 4), hDAT cells (open bars; n = 5), hDAT A559V S/A cells (sparse diagonal striped bars; n = 6), hDAT A559V cells pretreated for 20 min with 1 μm raclopride (dense diagonal striped bars; n = 4), hDAT A559V cells pretreated for 20 min with 5 μm antCaMKIINtide (sparse horizontal striped bars; n = 4), and hDAT S/D cells (dense horizontal striped bars; n = 5) (F(5,20) = 5.9, p = 0.0016, **p < 0.01, one-way ANOVA followed by Dunnett's test compared to hDAT A559V).