Fig 5.

Intranasal recombinant proteinase 2A and 3C–challenged mice show increased airway allergic phenotype. Age- and sex-matched C57/BL6 mice (n = 5 in each group) were immunized intranasally with OVA and recombinant proteinase 2A, proteinase 3C, buffer, or PBS every 2 days for a total of 16 days and 24 hours after the last immunization (A), and mice were assessed for airway hyperreactivity, which is shown as dose response to acetylcholine and using PC₂₀₀(B), and BAL and differential cells (C). Lung-specific IL-4 and IFN-γ ELISpot (D), OVA-specific IgG1 levels in serum (E), and lung lavage glycoprotein levels (F) were determined from the same groups of mice. ^∗P < .05 relative to PBS and buffer and ^∗∗P < .05 relative to proteinase 3C, PBS, and buffer by using 1-way ANOVA and the t test. Data represent means ± SDs. Concentrations of IL-4 (G), IL-5 (H), IFN-γ (I), IL-17 (J), CXCL-10 (K), and TNF (L) were measured in supernatants of single-cell cultures of lung cells by using Luminex. ^∗P < .05 relative to buffer and ^∗∗P < .05 relative to proteinase 3C and buffer by using 1-way ANOVA and the t test. Data are representative of 3 independent experiments; values shown are means ± SDs.