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a. Determination of the precise miRNA sequence by 5′ and 3′ miR-RACE. (a) miRNA cDNA library construction. (b) Analysis of miRNA 5′RACE and 3′RACE. Sequences for the 5′RNA adaptor, dT(30)RT primer, MirRacer 3′ Primer, MirRacer 5′ Primer, GSP1, and GSP2 are listed in Table 2.
