Table 1. Electrophysiological properties of wild-type and mutant hNa_v1.5 channels in HEK293 cells.

Channel	peak currrent	Steady-state activation			Steady-state inactivation			Recovery from inactivation
	density (pA/pF)	k (mV)	V1/2 (mV)	n	k (mV)	V1/2 (mV)	n	τf (ms)	Af	τs (ms)	As	n
hNav1.5	−866.5±79.8	6.9±0.2	−34.7±0.7	23	−6.4±0.1	−81.4±0.7	24	6.9±0.3	0.89±0.01	149.2±11.8	0.11±0.01	20
Group 1 mutants
L212P	−805.3±76.7	8.0±0.3▴	−49.7±0.8▴	22	−5.5±0.1▴	−91.9±0.8▴	28	7.5±0.4	0.86±0.02	159.9±12.1	0.14±0.02	19
P1298L	−789.6±84.0	7.2±0.2	−35.2±0.6	19	−6.8±0.1*	−90.3±0.6▴	24	7.4±0.3	0.90±0.01	194.6±17.0	0.10±0.01	22
DelF1617	−687.8±64.9	7.9±0.2▴	−41.5±1.2▴	24	−6.7±0.1	−92.0±1.1▴	25	6.0±0.4*	0.80±0.01▴	108.4±8.39*	0.19±0.01▴	18
R1632H	−1105.6±112.0	7.0±0.2	−36.1±0.8	21	−7.2±0.1▴	−102.1±0.9▴	25	114.3±11.8▴	0.28±0.02▴	695.8±36.4▴	0.72±0.02▴	23
Group 2 mutants
E161K	−203.7±30.8▴	9.0±0.1▴	−14.9±0.7▴	19	−6.6±0.1	−79.9±0.9	21	6.5±0.4	0.86±0.02	192.3±47.8	0.12±0.02	14
T220I	−533.8±56.6*	7.3±0.1	−35.7±0.7	25	−6.5±0.1	−87.0±0.8▴	30	8.0±0.4*	0.86±0.01	123.2±8.8	0.137±0.01	21
D1275N	−575.0±57.6*	6.8±0.2	−31.6±0.8▴	18	−5.5±0.1▴	−79.6±0.6	21	3.9±0.1▴	0.89±0.01	140.4±10.4	0.107±0.01	23

*indicates p<0.05 versus hNa_v1.5.

^▴indicates p<0.01 versus hNa_v1.5.