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. 2010 Jun 7;5(6):e10998. doi: 10.1371/journal.pone.0010998

Figure 4. In vitro hypertrophy and degradation of cartilage, and bone replacement.

Figure 4

(A) The morphology of aggregate cultured at day 50. Scale bars represent 100 µm. (B) The histological section of aggregate cultured at day 50 or 60 was stained by H&E. Hypertrophic cartilage (a), Degraded cartilage (b), Degraded cartilage and bone replacement (c) and bone replacement (d). Scale bars represent 20 µm. (C) Apoptotic cells were indicated by TUNEL staining. TUNEL (a), Bright field (b). Scale bars represent 20 µm. (D)The section of bone replacement cultured at day 60. H&E (a), Alcian Blue (b), Alizarin Red S (c), Alcian Blue & Alizarin Red S (d), Methylene Blue (e). Scale bars represent 20 µm. (E) The section of bone cultured at day 100. H&E (a), Alizarin Red S (b), COL 1 (c). Scale bars represent 20 µm.